BactoReal® Kit Mycobacterium avium ssp. paratuberculosis is a real-time PCR assay for detection of M. avium ssp. paratuberculosis (MAP) DNA. This test was developed and validated for the ABI PRISM® 7500 (Fast) instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® (Agilent), but is also suitable for other real-time PCR instruments. This test allows the rapid and sensitive detection of DNA of M. avium ssp. paratuberculosis purified from fecal samples, milk or tissues (e.g. with the QIAamp DNA Mini Kit extraction methods).