BactoReal® Kit Clostridium perfringens is a real-time PCR assay for detection the DNA of all five serotypes A-E of Clostridium perfringens. It is based on the amplification and detection of the 16S rRNA gene of C. perfringens. This test was developed and validated for the ABI PRISM® 7500 (Fast) instrument (Life Technologies), LightCycler® 480 (Roche), and for Mx3005P® (Agilent) but is also suitable for other real-time PCR instruments. This test allows the rapid and sensitive detection of DNA of Clostridium perfringens from samples purified from stool, tissue, etc (e.g. with the QIAamp DNA Mini Kit).