BactoReal® Typing Kit E. coli contains PCR primer sets for the amplification and detection of porcine E. coli virulence-associated genes. The method is based on an intercalating dye using real-time PCR. Amplification curve and gene specific melting curve at 515 nm indicate the amplification of E. coli specific genes. Screening for porcine E. coli adhesion and toxin genes requires picking of several bacterial colonies per plate. With one BactoReal® Typing Kit E. coli kit 20 colonies (2 x 96 wells) can be typed.