BactoReal® Kit Borrelia burgdorferi sensu lato is a real-time PCR assay for detection of DNA of Borrelia species belonging to the Borrelia burgdorferi sensu lato complex, namely Borrelia burgdorferi sensu stricto, B. garinii, B. afzelii, B. valaisiana, B. lusitaniae, B. bavariensis, B. bissettii and B. spielmanii. Borrelia spielmanii is not well detected with this assay. Borrelia causing relapsing fever are not detected.
This test was developed and validated for the ABI PRISM® 7500 (Fast) instrument (Life Technologies),
LightCycler® 480 (Roche) and Mx3005P® (Agilent), but is also suitable for other real-time PCR instruments. This test allows the rapid and sensitive detection of Borrelia burgdorferi sensu lato strains from samples purified from tissue, blood or cerebrospinal fluid (e.g. with the QIAamp DNA Mini Kit).